Immunoprecipitation of Cell Surface Proteins from Gram-negative Bacteria

Immunoprecipitation of Cell Surface Proteins from Gram-negative Bacteria

Blog Article

The meningococcus (Neisseria meningitidis) remains an important threat to human health worldwide.This Gram-negative bacterium causes elevated disabilities and mortality in infected individuals.Despite several available vaccines, currently there is no universal vaccine against all circulating meningococcal strains (Vogel et al.

, 2013).Herein, we Diffuser describe a new protocol that is capable of identifying only cell surface exposed proteins that play a role in immunity, providing this research field with a more straightforward approach to identify novel vaccine targets.Even though N.

meningitidis is used as a model in the protocol herein described, this protocol can be used for any Gram-negative bacteria provided modifications and optimizations are carried Outdoor Cooler out to adapt it to different bacterial and disease characteristics (e.g., membrane fragility, growth methods, serum antibody levels, etc.

).